Review



4x laemmli sample buffer bio rad  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier
Bioz Manufacturer Symbol Bio-Rad manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Bio-Rad 4x laemmli sample buffer bio rad
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    4x Laemmli Sample Buffer Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 11850 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+buffer/4x+Laemmli+Sample+Buffer/pmc13675934-66-17-21
    Average 99 stars, based on 11850 article reviews
    4x laemmli sample buffer bio rad - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Elucidating the Molecular Impact of DA29‐Induced ClpP Chemoactivation on Mitochondrial Function"

    Article Title: Elucidating the Molecular Impact of DA29‐Induced ClpP Chemoactivation on Mitochondrial Function

    Journal: The FASEB Journal

    doi: 10.1096/fj.202603398RR

    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, Bio‐Rad) are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    Figure Legend Snippet: Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, Bio‐Rad) are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.

    Techniques Used: Activation Assay, Western Blot, Control, Analysis, Gradient Centrifugation, Migration, Marker, Fluorescence, Activity Assay, Incubation, Concentration Assay, Molecular Weight, Staining, Negative Control

    Related Articles

    Western Blot:

    Article Title: Beyond binary: mapping the evolution of melanoma across a discrete gradient of acquired chemoresistance
    Article Snippet: For flow cytometry: Verapamil (Ambeed), 2-NBDG (ThermoFisher), DRB18 (Sigma-Aldrich), DMEM without glucose (ThermoFisher), Glucose solution (ThermoFisher), Attune NxT (ThermoFisher). .. For western blots: 1% Triton-X Lysis buffer (ThermoFisher), protease inhibitor cocktail (Thermofisher), BCA assay (ThermoFisher), electrophoresis equipment (BioRad), protein ladder (ThermoFisher), PVDF membranes (BioRad), 4X Laemmli buffer (BioRad), nonfat dried milk (RPI), insulin solution (ThermoFisher), primary and secondary antibodies (abcam), HRP substrate (ThermoFisher), BioRad ChemiDoc MP (BioRad). .. For immunofluorescence: 22 x 22 mm coverslips (Fisher Scientific), Cytofix / Cytoperm kit (BD Biosciences), BSA (Glentham Life Sciences), primary and secondary antibodies (abcam), Phalloidin-iFluor 594 (AAT Bioquest), Fluoromount-G with DAPI (SouthernBiotech), ZEISS LSM 710 confocal microscope (ZEISS), BioTek Lionheart FX (BioTek).

    Lysis:

    Article Title: Beyond binary: mapping the evolution of melanoma across a discrete gradient of acquired chemoresistance
    Article Snippet: For flow cytometry: Verapamil (Ambeed), 2-NBDG (ThermoFisher), DRB18 (Sigma-Aldrich), DMEM without glucose (ThermoFisher), Glucose solution (ThermoFisher), Attune NxT (ThermoFisher). .. For western blots: 1% Triton-X Lysis buffer (ThermoFisher), protease inhibitor cocktail (Thermofisher), BCA assay (ThermoFisher), electrophoresis equipment (BioRad), protein ladder (ThermoFisher), PVDF membranes (BioRad), 4X Laemmli buffer (BioRad), nonfat dried milk (RPI), insulin solution (ThermoFisher), primary and secondary antibodies (abcam), HRP substrate (ThermoFisher), BioRad ChemiDoc MP (BioRad). .. For immunofluorescence: 22 x 22 mm coverslips (Fisher Scientific), Cytofix / Cytoperm kit (BD Biosciences), BSA (Glentham Life Sciences), primary and secondary antibodies (abcam), Phalloidin-iFluor 594 (AAT Bioquest), Fluoromount-G with DAPI (SouthernBiotech), ZEISS LSM 710 confocal microscope (ZEISS), BioTek Lionheart FX (BioTek).

    Protease Inhibitor:

    Article Title: Beyond binary: mapping the evolution of melanoma across a discrete gradient of acquired chemoresistance
    Article Snippet: For flow cytometry: Verapamil (Ambeed), 2-NBDG (ThermoFisher), DRB18 (Sigma-Aldrich), DMEM without glucose (ThermoFisher), Glucose solution (ThermoFisher), Attune NxT (ThermoFisher). .. For western blots: 1% Triton-X Lysis buffer (ThermoFisher), protease inhibitor cocktail (Thermofisher), BCA assay (ThermoFisher), electrophoresis equipment (BioRad), protein ladder (ThermoFisher), PVDF membranes (BioRad), 4X Laemmli buffer (BioRad), nonfat dried milk (RPI), insulin solution (ThermoFisher), primary and secondary antibodies (abcam), HRP substrate (ThermoFisher), BioRad ChemiDoc MP (BioRad). .. For immunofluorescence: 22 x 22 mm coverslips (Fisher Scientific), Cytofix / Cytoperm kit (BD Biosciences), BSA (Glentham Life Sciences), primary and secondary antibodies (abcam), Phalloidin-iFluor 594 (AAT Bioquest), Fluoromount-G with DAPI (SouthernBiotech), ZEISS LSM 710 confocal microscope (ZEISS), BioTek Lionheart FX (BioTek).

    BIA-KA:

    Article Title: Beyond binary: mapping the evolution of melanoma across a discrete gradient of acquired chemoresistance
    Article Snippet: For flow cytometry: Verapamil (Ambeed), 2-NBDG (ThermoFisher), DRB18 (Sigma-Aldrich), DMEM without glucose (ThermoFisher), Glucose solution (ThermoFisher), Attune NxT (ThermoFisher). .. For western blots: 1% Triton-X Lysis buffer (ThermoFisher), protease inhibitor cocktail (Thermofisher), BCA assay (ThermoFisher), electrophoresis equipment (BioRad), protein ladder (ThermoFisher), PVDF membranes (BioRad), 4X Laemmli buffer (BioRad), nonfat dried milk (RPI), insulin solution (ThermoFisher), primary and secondary antibodies (abcam), HRP substrate (ThermoFisher), BioRad ChemiDoc MP (BioRad). .. For immunofluorescence: 22 x 22 mm coverslips (Fisher Scientific), Cytofix / Cytoperm kit (BD Biosciences), BSA (Glentham Life Sciences), primary and secondary antibodies (abcam), Phalloidin-iFluor 594 (AAT Bioquest), Fluoromount-G with DAPI (SouthernBiotech), ZEISS LSM 710 confocal microscope (ZEISS), BioTek Lionheart FX (BioTek).

    Article Title: Childhood trauma in women with HIV is associated with increased mitochondrial respiration despite reduced mitochondrial DNA copy number.
    Article Snippet: PBMCs were pelleted and homogenized with RIPA buffer (Thermo Fisher, 89900) supplemented with 1% protease inhibitor (Sigma-Aldrich, 2714). .. Cells were quantified by BCA assay (Thermo Fisher Scientific, 23227), and samples were prepared in 4x Laemmli buffer (Bio Rad, 1610747) and 10% β-Mercaptoethanol for a final concentration of 5ug/30μL. ..

    Electrophoresis:

    Article Title: Beyond binary: mapping the evolution of melanoma across a discrete gradient of acquired chemoresistance
    Article Snippet: For flow cytometry: Verapamil (Ambeed), 2-NBDG (ThermoFisher), DRB18 (Sigma-Aldrich), DMEM without glucose (ThermoFisher), Glucose solution (ThermoFisher), Attune NxT (ThermoFisher). .. For western blots: 1% Triton-X Lysis buffer (ThermoFisher), protease inhibitor cocktail (Thermofisher), BCA assay (ThermoFisher), electrophoresis equipment (BioRad), protein ladder (ThermoFisher), PVDF membranes (BioRad), 4X Laemmli buffer (BioRad), nonfat dried milk (RPI), insulin solution (ThermoFisher), primary and secondary antibodies (abcam), HRP substrate (ThermoFisher), BioRad ChemiDoc MP (BioRad). .. For immunofluorescence: 22 x 22 mm coverslips (Fisher Scientific), Cytofix / Cytoperm kit (BD Biosciences), BSA (Glentham Life Sciences), primary and secondary antibodies (abcam), Phalloidin-iFluor 594 (AAT Bioquest), Fluoromount-G with DAPI (SouthernBiotech), ZEISS LSM 710 confocal microscope (ZEISS), BioTek Lionheart FX (BioTek).

    SDS Page:

    Article Title: Mustn1b is required for cardiomyocyte dedifferentiation and heart regeneration in zebrafish.
    Article Snippet: 25 Unlike the adult mammalian heart, the adult zebrafish heart can robustly regenerate via 26 processes that include cardiomyocyte (CM) dedifferentiation, proliferation, and repopulation 27 of the damaged tissue.. Although CM dedifferentiation is considered indispensable for 28 cardiac regeneration, the factors driving it remain elusive.. Here, we examine the role of 29 Mustn1b in CM dedifferentiation during zebrafish cardiac regeneration.

    Article Title: Truncated ErbB2 receptor expression lowers nucleus stiffness and increases migration of breast cancer cells
    Article Snippet: Protein concentration was measured using the Pierce BCA protein assay kit. .. Protein lysates were mixed with 4x Laemmli buffer containing DTT and boiled at 95∘C for 5 min. Proteins were separated by SDS-PAGE on 4-15% TGX Stain-Free gels and transferred to PVDF membranes using a semidry transfer system (Trans-Blot Turbo, Bio-Rad). ..

    Membrane:

    Article Title: Mustn1b is required for cardiomyocyte dedifferentiation and heart regeneration in zebrafish.
    Article Snippet: 25 Unlike the adult mammalian heart, the adult zebrafish heart can robustly regenerate via 26 processes that include cardiomyocyte (CM) dedifferentiation, proliferation, and repopulation 27 of the damaged tissue.. Although CM dedifferentiation is considered indispensable for 28 cardiac regeneration, the factors driving it remain elusive.. Here, we examine the role of 29 Mustn1b in CM dedifferentiation during zebrafish cardiac regeneration.

    Incubation:

    Article Title: A bare host cell membrane with minimal glycocalyx is an optimal surface for targeting by virulence-primed Salmonella Typhimurium
    Article Snippet: A 1:10 dilution of the samples was used to determine protein concentrations in a bicinchoninic acid assay (Thermo Fisher) using dilutions of Pierce TM BSA standard (Thermo Fisher) for reference. .. 20 μg of protein were mixed with 4x Laemmli buffer (Biorad) containing 5% β-mercaptoethanol, incubated for 5 min at 95°C and loaded on a 4–20% Mini-PROTEAN® TGX Stain-FreeTM Protein Gel (Biorad) along with 5 μL of ladder (Biorad). .. Gels were run for 35 min at 200 V and transferred to a nitrocellulose membrane (0.2 μm pore size; Biorad) with a Trans-Blot Turbo Transfer System (Biorad) using the program for high molecular weight.

    Staining:

    Article Title: A bare host cell membrane with minimal glycocalyx is an optimal surface for targeting by virulence-primed Salmonella Typhimurium
    Article Snippet: A 1:10 dilution of the samples was used to determine protein concentrations in a bicinchoninic acid assay (Thermo Fisher) using dilutions of Pierce TM BSA standard (Thermo Fisher) for reference. .. 20 μg of protein were mixed with 4x Laemmli buffer (Biorad) containing 5% β-mercaptoethanol, incubated for 5 min at 95°C and loaded on a 4–20% Mini-PROTEAN® TGX Stain-FreeTM Protein Gel (Biorad) along with 5 μL of ladder (Biorad). .. Gels were run for 35 min at 200 V and transferred to a nitrocellulose membrane (0.2 μm pore size; Biorad) with a Trans-Blot Turbo Transfer System (Biorad) using the program for high molecular weight.

    Article Title: Truncated ErbB2 receptor expression lowers nucleus stiffness and increases migration of breast cancer cells
    Article Snippet: Protein concentration was measured using the Pierce BCA protein assay kit. .. Protein lysates were mixed with 4x Laemmli buffer containing DTT and boiled at 95∘C for 5 min. Proteins were separated by SDS-PAGE on 4-15% TGX Stain-Free gels and transferred to PVDF membranes using a semidry transfer system (Trans-Blot Turbo, Bio-Rad). ..

    Concentration Assay:

    Article Title: Childhood trauma in women with HIV is associated with increased mitochondrial respiration despite reduced mitochondrial DNA copy number.
    Article Snippet: PBMCs were pelleted and homogenized with RIPA buffer (Thermo Fisher, 89900) supplemented with 1% protease inhibitor (Sigma-Aldrich, 2714). .. Cells were quantified by BCA assay (Thermo Fisher Scientific, 23227), and samples were prepared in 4x Laemmli buffer (Bio Rad, 1610747) and 10% β-Mercaptoethanol for a final concentration of 5ug/30μL. ..



    Similar Products

    4x laemmli sample buffer bio rad  (Bio-Rad)
    99
    Bio-Rad 4x laemmli sample buffer bio rad
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    4x Laemmli Sample Buffer Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+buffer/4x+Laemmli+Sample+Buffer/pmc13675934-66-17-21
    Average 99 stars, based on 1 article reviews
    4x laemmli sample buffer bio rad - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    1x laemmli sample buffer  (Bio-Rad)
    99
    Bio-Rad 1x laemmli sample buffer
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    1x Laemmli Sample Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+buffer/4x+Laemmli+Sample+Buffer/pm42889706-1016-16-20
    Average 99 stars, based on 1 article reviews
    1x laemmli sample buffer - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    laemmli sample buffer  (Bio-Rad)
    99
    Bio-Rad laemmli sample buffer
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    Laemmli Sample Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+buffer/4x+Laemmli+Sample+Buffer/pm42883798-169-13-17
    Average 99 stars, based on 1 article reviews
    laemmli sample buffer - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    4x laemmli sample buffer  (Bio-Rad)
    99
    Bio-Rad 4x laemmli sample buffer
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    4x Laemmli Sample Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+buffer/4x+Laemmli+Sample+Buffer/pm42886259-33-4-27
    Average 99 stars, based on 1 article reviews
    4x laemmli sample buffer - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    protein concentrations  (Bio-Rad)
    99
    Bio-Rad protein concentrations
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    Protein Concentrations, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+buffer/4x+Laemmli+Sample+Buffer/pm42886558-335-3-14
    Average 99 stars, based on 1 article reviews
    protein concentrations - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    sample buffer  (Bio-Rad)
    99
    Bio-Rad sample buffer
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    Sample Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+buffer/4x+Laemmli+Sample+Buffer/pm42882515-76-8-11
    Average 99 stars, based on 1 article reviews
    sample buffer - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    laemmli buffer  (Bio-Rad)
    99
    Bio-Rad laemmli buffer
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    Laemmli Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+buffer/4x+Laemmli+Sample+Buffer/pm42884018-299-9-11
    Average 99 stars, based on 1 article reviews
    laemmli buffer - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    4x laemmli buffer  (Bio-Rad)
    99
    Bio-Rad 4x laemmli buffer
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    4x Laemmli Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4x+laemmli+buffer/4x+Laemmli+Sample+Buffer/pm42880380-103-15-18
    Average 99 stars, based on 1 article reviews
    4x laemmli buffer - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, Bio‐Rad) are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.

    Journal: The FASEB Journal

    Article Title: Elucidating the Molecular Impact of DA29‐Induced ClpP Chemoactivation on Mitochondrial Function

    doi: 10.1096/fj.202603398RR

    Figure Lengend Snippet: Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, Bio‐Rad) are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.

    Article Snippet: Whole‐cell proteins (20‐30 μg), mitochondrial lysates (30 μg) or sucrose gradient fractions (10 μL) were denatured in 4X Laemmli sample buffer (Bio‐Rad) complemented with 10% 2‐mercaptoethanol, separated by SDS‐PAGE and subjected to immunoblotting as described in [ ], using the following primary antibodies: ClpP (#A306224, Antibodies.com), ClpX (#ab168338, Abcam), total OXPHOS cocktail (#ab110413, Abcam), HA‐tag (#3724, Cell Signaling Technology), uL13m (#16241–1‐AP, Proteintech), VDAC1 (#ab186321, Abcam), mS29 (# MA141279 , Thermo Fisher Scientific), α‐tubulin (#T6074, Sigma‐Aldrich) and β‐actin (#A2066, Sigma‐Aldrich).

    Techniques: Activation Assay, Western Blot, Control, Analysis, Gradient Centrifugation, Migration, Marker, Fluorescence, Activity Assay, Incubation, Concentration Assay, Molecular Weight, Staining, Negative Control